Host mRNA abundance of genes of interest (fold change infected/uninfected).

mRNA fold change of host genes of interest in was calculated for infected cells versus uninfected cells. RNA samples were isolated from infected dishes paired to infected metabolomics dishes and from an uninfected control dish. RNAseq was performed to generate the transcriptome and fold change (infected/uninfected) determined. Infection increased expression of multiple host metabolic genes in central pathways including glycolysis, the PPP, nucleotide metabolism, and amino acid synthesis and transport. Large amino acid transporter 1 (LAT1), argininosuccinate synthase 1 (ASS1), phosphoglycerate dehydrogenase (PHGDH), methylenetetrahydrofolate dehydrogenase (MTHFD1), 5-phosphoribosyl-1-pyrophosphate synthase (PRS1), hexose kinase (HK), pyruvate kinase (PK), fructose-1,6-bisphosphate aldolase 1 (FBA1), lactate dehydrogenase A (LDHA), bisphosphoglycerate mutase (BPGM), aconitase (ACN), isocitrate dehydrogenase (IDH), alpha-ketoglutarate dehydrogenase (A-KGDH), succinate reductase (SCR), fumarase (FUM), malate dehydrogenase (MDH), citrate synthase (CS), succinyl-CoA synthetase (SCS), glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconolactonase (6PGL), 6-phosphogluconate dehydrogenase (6PGD), ribose-5-phosphate isomerase (R5PI), ribulose-5-phosphate epimerase (Ru5PE), transaldolase (TA), transketolase (TK).