The Modulation of Chaihu Shugan Formula on Microbiota Composition in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) Technology Platform and Its Influence on Gut Barrier and Intestinal Immunity in Caco-2/THP1-blue Cell Co-culture M

we investigate the influence of CSF on the modulation of the simulated colonic microbiota of five healthy donors, gut barrier integrity, and intestinal immunity by combining the simulator of the human intestinal microbial ecosystem (SHIME) technology platform with co-culture of intestinal and immune cells.Ten samples were collected at the start (control condition only) and 20 samples after 48h incubation (all conditions) for microbial community analysis. DNA was isolated from a total of 30 samples according to the method described by Catalayud et al.. 16S-targeted Illumina sequencing was performed to obtain the microbiota profiling of the colon compartment.