Genetic background and diet induce hepatic gene expression of different regulatory pathways associated with obesity in the Collaborative Cross

In this study, we used 123 Collaborative Cross female mice fed either a high protein (HP) or high fat high sucrose (HS) diet to perform differential gene expression and analyses in the liver. We also determined the correlation of liver gene expression levels with phenotypic data of adiposity and obesity-related traits. Overall design: Female mice from 22 CC strains were obtained from University of North Carolina’s CC Mouse Core at 6-9 weeks of age. Mice were then acclimated for 2 weeks on a synthetic diet, housed 3 mice per cage. Then, mice were put on experimental diets, randomized into different cages by experimental diet, and housed 3-5 mice per cage. Mice were challenged on their respective diets for 8 full weeks and sacrificed the following week after a 4-hour fast and anesthetization to obtain liver samples for microarray analysis. Microarray data were available for 127 samples.

在本项研究中，我们选取了123只合作杂交雌性小鼠，分别喂养高蛋白（HP）或高脂肪高糖（HS）饮食，以在肝脏中执行差异基因表达分析。同时，我们还确定了肝脏基因表达水平与肥胖及相关表型数据之间的相关性。总体设计如下：从北卡罗来纳大学合作杂交小鼠核心实验室获取了22个CC品系的小鼠，其年龄在6-9周之间。随后，小鼠在合成饲料上适应了2周，每笼饲养3只。之后，小鼠被分配到不同的笼子中，并根据实验饮食进行随机分组，每笼饲养3-5只。小鼠在各自的饮食条件下进行了8周的挑战，并在接下来的星期牺牲，经过4小时的禁食和麻醉后，以获取肝脏样本用于微阵列分析。共有127个样本的微阵列数据可供使用。