Extraction of <i>R</i>. <i>typhi</i> DNA from normal human plasma spiked with <i>R</i>. <i>typhi</i> for evaluation of RPA-exo and RPA-nfo.

a The copy number of R. typhi 17 kDa gene was determined by qPCR (expressed as copy # per μL of DNA template) and presented as mean ± standard deviation of three replicates. 10 μL of the extracted DNA was used for RPA-exo or RPA-nfo detection. The expected copy number of 17 kDa gene present in each of the spiked sample was 8 copies per μL based on 2000 copies/mL of 200 μL of spiked NHP and assuming 100% recovery after DNA extraction. Extraction of R. typhi DNA from normal human plasma spiked with R. typhi for evaluation of RPA-exo and RPA-nfo.