Single-cell RNA sequencing of mouse primary B cells to investigate subpopulation markers

The project aims at linking functionality to metabolic fluxes of mouse primary B cells, potentially correlating antibody secretion rates with metabolic differences in subpopulations of B cells. In previous experiments, subpopulations of primary B cells with different phenotypes were observed. Therefore, the next step is to further characterize these subpopulations of B cells via single-cell RNA sequencing so that we gain insight about cell characteristics and individual gene markers, and their potential biological role. Our hypothesis is that these cells present different expression profiles of key genes and/or are relevant in different immune-related pathways.本项目旨在将功能与小鼠原代B细胞的代谢通量（metabolic fluxes）建立关联，以期将抗体分泌速率与B细胞亚群的代谢差异进行相关性分析。 既往实验中，研究人员已观测到具有不同表型的原代B细胞亚群。 因此，本研究的下一步计划是通过单细胞RNA测序（single-cell RNA sequencing）对上述B细胞亚群开展进一步表征，以深入解析细胞特性、单个基因标记物及其潜在的生物学作用。 本研究的假说为：这些细胞呈现出关键基因的差异化表达谱，或参与不同的免疫相关通路。