Bacillus cereus Genome sequencing and assembly

In an effort to better understand how Bacillus cereus acquires multidrug resistance, we examined the organism's response to elevated levels of different antibiotics: tetracycline, ciprofloxacin and vancomycin. In this study, we obtained eighteen isolates that showed resistance to selected antibiotics. Illumina and PacBio sequencing of the isolates and comparing them to the parental strain helped us to identify genomic changes during the stepwise in vitro generation of multidrug resistant isolates.