Offspring behavioral outcomes following maternal allergic asthma in the IL-4-deficient mouse

Background: Maternal allergies and asthma during pregnancy have been associated with increased risk of ASD and ADHD to the child. Previous rodent studies have demonstrated that inducing a T helper-2 (Th2)-mediated allergic response during pregnancy leads to an offspring behavioral phenotype characterized by decreased social interaction and increased stereotypies. Interleukin-4 is a key signal in the Th2 immune cascade, but its role in fetal brain development and subsequent impacts of maternal allergic asthma (MAA) on offspring behavioral deficits have yet to be determined.
Objective: In this study, we investigated whether the absence of IL-4 signaling would mitigate MAA-induced behavioral changes.
Methods: C57BL/6J and Interleukin-4 knockout (IL-4 KO) mice were sensitized to ovalbumin and exposed to repeated allergic asthma aerosol inductions throughout pregnancy. Offspring were assessed on Juvenile Reciprocal Social Interaction, Elevated Plus Maze, Open Field Exploration, Novel Object ..., Animals
            Male and female C57Bl/6-Il4tm1Nnt/J mice (IL-4 KO; stock #002518) and wild type mice C57Bl/6J (C57; stock #000664) were purchased from Jackson Laboratory (Bar Harbor, MA, USA), bred at Mount Holyoke College, South Hadley, MA, and maintained at ambient room temperature on a 12 h light/dark cycle (lights on at 0800 h). Mice were group-housed in individually ventilated cages with same-sex littermates until breeding at 8-weeks of age. Cages were maintained in a temperature-controlled (23° C) vivarium with food and water provided ad libitum. All procedures were performed with approval by the Mount Holyoke College Institutional Animal Care and Use Committee and in accordance with the guidelines provided by the National Institutes of Health Guide for the Care and Use of Laboratory Animals.
Maternal allergic asthma inductions
            Allergic asthma inductions were carried out using procedures previously described (Schwartzer et al., 2015; Schwartzer et al., 2017). Sexua..., , # Offspring behavioral outcomes following maternal allergic asthma in the IL-4-deficient mouse

GENERAL INFORMATION

1. Title of Dataset: Maternal serum analysis and offspring behavioral outcomes following maternal allergic asthma in C57Bl/6J and IL-4 deficient mince.

2. Author Information
Corresponding Author
Name: Jared Schwartzer
Institution: Mount Holyoke College
Address: 50 College Street, South Hadley, MA USA
Email: [[e-mail]](mailto:[e-mail])

3. Data of data collection: 2021-2023

4. Funding Source: National Institutes of Health, R15MH119500.

DATA & FILE OVERVIEW

1. File List:

A) Behavior Data.csv

B) Maternal Serum.csv

1. Relationship between files, if important: Maternal Serum ID is Litter ID on Behavior Data.csv

2. Additional related data collected that was not included in the current data package: None

3. Abbreviations in Datasets
JRSI - Juvenile Reciprocal Social Interaction
EPM - Elevated Plus Maze
...