Information on 24 tropical trees subjected to next-generation sequencing and screened for SSRs.

aBased on the Agroforestree Database (www.worldagroforestry.org/resources/databases/agroforestree), an open access resource of ICRAF that provides data on >650 trees.bThe seed source of material for NGS varied and included natural stands, seed orchards and landraces. The numerical reference is the ICRAF accession number.cCurrent data from NGS; complete information is available at the tropiTree portal (http://bioinf.hutton.ac.uk/tropiTree). In () is the number of perfect SSRs identified. In [] is the percentage of the corresponding transcripts that have TAIR hits (for all SSRs).dData from National Center for Biotechnology Information of the USA (NCBI) searches were included to illustrate previous sequencing work. Searches were undertaken on 14 April 2014 via the Entrez search system (www.ncbi.nlm.nih.gov/sites/gquery). Species names for NCBI searches were checked as correct against current nomenclature using the Agroforestry Species Switchboard (www.worldagroforestry.org/products/switchboard/), an open access resource of ICRAF that provides links to information on >20,000 plants. Current names were set as ‘organism’ in NCBI searches. In () is the number of ESTs listed in NCBI nucleotide citations (if any). In [] is the number of NGS studies cited in NCBI’s Sequence Read Archive (if any).eAs well as being of importance to small-scale farmers, Acacia mangium and Jatropha curcas have wide commercial interests (see text), explaining the high NCBI citations.fSpecies were subject to primer validation (see text).