Restriction-site associated DNA (RAD) tag sequencing in 213 individual birches from the United Kingdom. Betula RAD-seq from Britain
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https://www.ncbi.nlm.nih.gov/bioproject/PRJEB3322
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Plant tissues (apart from one individual where DNA extracted for whole genome sequencing was used (see study accession ERP001867)) were collected in the field from plants identified by morphology as B. nana (38 individuals in addition to the one used for WGS in ERP001867), B. pubescens (137 individuals), B. pendula (37 individuals) and B. × intermedia (one individual). Genomic DNA was extracted and RAD libraries were prepared for all individuals using restriction enzyme PstI. These were sequenced on an Illumina HiSeq 2000 instrument at the GenePool genomics facility in the University of Edinburgh, using 100 bp single end reads and 50 bp paired-end reads. To match the read files to populations and locations, look at the "Submitted FTP" file name. The first set of letters/numbers in these file names correspond to the Sample IDs given in Table S1 of Zohren et al 2016 Mol. Ecol..
创建时间:
2012-11-21



