A combinatorial Interplay Among the ACC Synthase Isoforms Regulates Ethylene Biosynthesis in Arabidopsis thaliana

ACC Synthase (ACS) is the key regulatory enzyme in the ethylene biosynthesis in plants. It catalyzes the conversion of s-adenosylmethionine (SAM) to 1-aminocyclopropane-1-carboxylic acid (ACC), the precursor of ethylene. Arabidopsis has nine ACS genes. The goal of the project is to inactivate each gene by insertional mutagenesis and amiRNA technology and eventually construct a null ACS mutant. We have been recently able to achieve this goal. Furthermore, we wanted to know how inactivation of individual ACS genes affects global gene expression. Keywords: ACS mutant comprrison; global gene expression. Overall design: Triplicate samples of 10-day light grown seedling from each ACS mutant was used for microarray analysis.

ACC合酶（ACC Synthase）是植物中乙烯生物合成过程中的关键调控酶。该酶催化S-腺苷甲硫氨酸（s-adenosylmethionine，SAM）转化为1-氨基环丙烷-1-羧酸（1-aminocyclopropane-1-carboxylic acid，ACC），ACC是乙烯的前体。拟南芥（Arabidopsis）拥有九个ACS基因。本项目旨在通过插入突变和amiRNA技术使每个基因失活，并最终构建一个无活性（null）的ACS突变体。近期，我们已经实现了这一目标。此外，我们希望了解单个ACS基因的失活如何影响全局基因表达。关键词：ACS突变体比较；全局基因表达。总体设计：每个ACS突变体的10天光照生长幼苗的重复样品（三重复）被用于微阵列分析。