Michael Cammer, Phyllis Novikoff (2011) CIL:35281, Rattus rattus, hepatocyte. CIL. Dataset

Primary rat hepatocytes in culture fixed and stained for beta-tubulin. The microtubules form a network throughout the cytoplasm. In this image they are seen originating from a brighter and denser region adjacent to the cell nucleus.

This is a single optical section a few microns up from the substrate collected by widefield fluorescence microscopy with a Photometrics camera with a KAF1400 chip. The imaging was performed at a microscopy course at Woods Hole Oceanographic Institute in October 1993 and deconvolved on VayTec software running on a Macintosh computer. At the time, deconvolution was new and novel for use by biologists.

培养的初级大鼠肝细胞经beta-微管蛋白固定和染色。微管在细胞质中形成网络。在此图像中，它们可见于紧邻细胞核的更亮、更密集区域。此图像为从基底板上方几微米处采集的单一光学切片，采用宽场荧光显微镜及配备KAF1400芯片的Photometrics相机拍摄。成像于1993年10月在伍兹霍尔海洋研究所的显微镜课程中进行，并使用运行在Macintosh计算机上的VayTec软件进行去卷积处理。当时，去卷积对于生物学家而言尚属新颖且具有创新性。