five

Platelet_ion_monitoring_Fig2

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To determine adequate cytosolic dye loading conditions. We incubated platelets with different concentrations of AM ester indicator derivatives (Fluo-3, ING-2, and IPG-2). Fluorescence was monitored and measured over time. Conclusion: This experiment allowed to determine optimal incubation time for the three indicators, and the optimal concentration with good fluorescence shifts and stable signals. Notes: Figure 2 FCS files from manuscript "Flow cytometric monitoring of dynamic cytosolic calcium, sodium, and potassium fluxes following platelet activation"
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2020-06-01
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