Comparative Chemical Fingerprinting of <i>Oroxylum Indicum</i> and <i>Scutellaria Baicalensis</i> Using Liquid Chromatography &amp; Mass Spectrometry

<b>Introduction</b>: Identification of <i>Oroxylum indicum</i> and <i>Scutellaria baicalensis</i> provide an interesting challenge in selection of biomarker compound to be used in routine analysis. Both plants have similar phytochemical profile and are rich source flavones and flavon- glycosides. <b>Objective</b>: To prepare the chemical fingerprinting of <i>Oroxylum indicum</i> bark and <i>Scutellaria baicalensis</i> roots using the Liquid chromatography and Mass Spectroscopy using single chromatographic method. <b>Methods and Material</b>- Extracts prepared using various solvent system (methanol, Aq methanol, chloroform, hexane and water) of both plants were analyzed using C18 reverse phase column with solvent system containing acetonitrile and 0.1% formic acid. Major Flavonoids were identified based on mass spectra, fragmentation pattern and UV spectra. <b>Results</b>: In this article well resolved HPLC separation in both plant extracts was obtained and chemical fingerprints for both plant extracts were established and flavonoids present(Baicalin, Oroxylin A -7-O-glucuronide,Chrysin-7-O-glucuronide, Baicalein, Chrysin, Oroxylin-A, Wogonin, Skullcap Flavone II) were identified as possible biomarkers. <b>Conclusion</b>: Mass spectrometry coupled with high performance liquid chromatography can be a tool for fingerprinting of various natural products used in dietary supplement industry. The fingerprint developed in the article can be used for quality evaluation as well as identifying possible adulteration of extracts of both the plants.