Sanger sequencing and ddPCR raw data files (A species-specific digital PCR assay for the endangered blue racer in Canada)

Manuscript title: A species-specific digital PCR assay for the endangered blue racer in Canada Authors: Orianne Tournayre, Ryan Wolfe, Hannah McCurdy-Adams, Amy A. Chabot and Stephen C.Lougheed Data: <strong>349360_chromats.zip</strong> (".ab1" files) : raw cytochrome b (cytb) sequences and chromatograms of 13 blue racer individuals collected on Pelee Island (Canada, see Table S1 for more information). Sequencing was done at the Centre for Applied Genomics (Toronto, Canada) using the L14910 (de Queiroz et al. 2002) and ColSeq1F (Burbrink et al. 2008) primers. ". ddpcr" files: ddPCR raw data generated using a Bio-Rad QX200™ AutoDG™Droplet Digital™ PCR System and direct quantification mode in the QX Manager 2.0 Standard Edition software (BioRad, Saint-Laurent, QC,Canada) <strong>In_vitro_data_ 20221214_145436_565.ddpcr</strong> : Standard concentration 1 Standard concentration 2: 1/2 dilution Standard concentration 3: 1/4 dilution Standard concentration 4: 1/8 dilution Standard concentration 5: 1/16 dilution Standard concentration 6: 1/32 dilution Standard concentration 7: 1/64 dilution Standard concentration 8: 1/128 dilution Standard concentration 9: 1/256 dilution Standard concentration 10: 1/512 dilution Standard concentration 11: 1/1024 dilution Standard concentration 12: 1/2048 dilution Standard concentration 13: 1/4096 dilution Standard concentration 14: 1/8192 dilution Standard concentration 15: 1/16384 dilution <strong>LOD_LOQ_data_20230113_145859_903.ddpcr</strong> <strong>In_situ_data_20221220_134107_549.ddpcr</strong>