Genes used in qRT-PCR assay.
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Details of the qRT-PCR compatible marker set selected by our combined filtering process. PCR efficiency was calculated based on the slope of the line after running a series of 10-fold dilutions of mixed-stage cDNA (Figure S2A). Limit of detection was calculated based on the number of parasite stages estimated to be present in the last dilution where the marker was detected. Detection limit ranged from 101–103 pg cDNA, corresponding to approximately 20–200 cells, depending on the stage.
创建时间：
2015-12-02
