Data Sheet 1_Impact of obstructive sleep apnea on gut microbiome of patients with symptomatic intracranial atherosclerotic stenosis.docx

IntroductionObstructive sleep apnea syndrome (OSAS) is positively associated with increased risks of ischemic stroke. Patients with stroke exhibit remarkable gut microbiota dysbiosis. However, the impact of OSAS on gut microbiota of patients with symptomatic intracranial atherosclerotic stenosis (sICAS), one of the most common causes of stroke, remains unknown.
MethodsThis study included patients with sICAS, the severity of OSAS was defined by the apnea-hypopnea index (AHI). AHI < 5 was considered no sleep apnea, AHI 5–15 was defined as mild OSAS, AHI 15–30 as moderate OSAS, and AHI > 30 as severe OSAS. Fecal samples were collected and subjected to 16 s rRNA gene sequencing. PICRUSt2 was used to predict the functional properties of the bacterial communities.
ResultsIn total, 99 sICAS patients were included, with No-OSAS (N = 22), Mild (N = 25), Moderate (N = 30), and Severe (N = 22). Patients with OSAS exhibited significantly altered gut microbiota composition compared to those without sleep apnea, characterized by increased abundances of pathogens such as Escherichia-Shigella and decreased abundances of beneficial microbes such as short-chain fatty acids-producing bacteria Blautia. Importantly, these microbes were significantly associated with AHI. Several microbial metabolic pathways such as Peptidoglycan biosynthesis, C5-branched dibasic acid metabolism, and Pantothenate and CoA biosynthesis were downregulated with OSAS.
ConclusionOSAS is associated with gut dysbiosis and altered microbial metabolic functions in patients with sICAS.