Dipsacales four locus supermatrix assembled with PyNCBIminer

PyNCBIminer is a user-friendly software that automates the assembly of large DNA datasets from GenBank for phylogenetic reconstruction using the supermatrix method.To evaluate the sensitivity of PyNCBIminer to initial queries, we downloaded four DNA markers (ITS, matK, rbcL and trnLintron-trnF) of Dipsacales using two distinct initial query sets. The experiments were performed on a personal computer featuring a 13th Gen Intel(R) Core (TM) i5-1340P 1.90 GHz processor and 32.0 GB of RAM. Run 1 used six well-aligned sequences from six angiosperm orders as default initial queries in PyNCBIminer, while Run 2 used six sequences from six genera within the order Dipsacales., , , # Dipsacales four locus supermatrix assembled with PyNCBIminer
[https://doi.org/10.5061/dryad.xpnvx0kq3](https://doi.org/10.5061/dryad.xpnvx0kq3)

## Description of the data and file structure

To evaluate the sensitivity of PyNCBIminer to initial queries, we downloaded four DNA markers (ITS, matK, rbcL and trnLintron-trnF) of Dipsacales using two distinct initial query sets. The experiments were performed on a personal computer featuring a 13th Gen Intel(R) Core (TM) i5-1340P 1.90 GHz processor and 32.0 GB of RAM. Run 1 used six well-aligned sequences from six angiosperm orders as default initial queries in PyNCBIminer, while Run 2 used six sequences from six genera within the order Dipsacales.

The zip file contained two folders for Run 1 and Run 2, which each contained the following sub directories or files:

\#original blast results for each maker named by the marker name, including 

*ITS, matK, rbcL, and trnL-trnF* are directly produced by the \"Sequence Retrieving\" module in PyNCB...