CIL:25823

Chromophore-assisted laser inactivation (CALI) inactivates target proteins by generating reactive photoproducts such as reactive oxygen species (ROS) by intense irradiation of photosensitizer chromophores, such as EGFP. Capping protein (CapZ, abbreviated CP throughout) is an obligate heterodimer of alpha and beta subunits and is the predominant actin filament capping protein in most cells. Rat2 cells expressing EGFP-CP, were subjected to CALI. After administration of a 100-ms dose of 1.5 mW/[μm]2 laser light, the dynamic changes on the dorsal surface of the cell seen in KDR cells (lacking endogenous CP and rescued with EGFP-CP, CIL# 25821,25822) were not observed in this DIC time-lapse movie. This and CIL 25824 are control movies. This movie is Movie3 in Supporting Information and still images are in Fig 2B, bottom panels, in Proc Natl Acad Sci (2007) 104: 6702-6707.