Transcriptomic analysis of the bone marrow from RNase L-/- mice following total body irradiation.

Ionizing radiation is capable of mediating damage on DNA, RNA, proteins and other intracellular biomolecules. The OAS/RNase L pathway, an intracellular RNA sensor that senses exogenous or endogenous dsRNA, plays an important role in the antiviral infection and innate immunity. RNase L is an essential endonuclease that regulates the degradation and translation of RNA during exogenous stimuli. Therefore, we hypothesized that ionizing radiation-induced RNA damage activates the RNA sensor in response to exogenous stresses. RNase L-/- mice were performed to explore the effect of RNase L in radiation-induced damage repair in the bone marrow, a radiation-sensitive organ. Bone marrow derived from RNase L-/- or wildtype mice, which were performed total-body irradiation at a dose of 6Gy by a 60Co irradiator, was analyzed for transcriptome. This experiment is divided into four groups: (1)Wildtype mice with non-treatment, (2)RNaseL-/- mice with non-treatment, (3)Wildtype mice exposed to irradiation, and (4) RNaseL-/- mice exposed to irradiation.