Bactrocera dorsalis strain:Punador Transcriptome or Gene expression

Comprehensive RNA sequencing was performed on a laboratory colony of B. dorsalis with a focus on attempting to capture as many genes in the sequencing from throughout the entire developmental life history. De novo assembly and analysis of the resulting sequence was focused on identifying full length gene and splice variants, and then annotating through identification of orthologs in Drosophila gene sets. In addition, differential expression analysis catalogued under what life history stages particular genes are over- or under-expressed, creating a high quality reference gene set that can be utilized in the laboratory to perform functional genomic experiments.

本研究对B. dorsalis的实验室菌株进行了全面RNA测序，旨在尽可能全面地捕捉其整个发育过程中的所有基因。对所得序列的从头组装与分析集中于识别全长基因及其剪接变体，并通过在果蝇基因集中识别同源基因进行注释。此外，差异表达分析按生命史阶段对特定基因的高表达或低表达进行了编目，构建了一个高质量的参考基因集，可供实验室进行功能基因组实验研究。