Antibiotics treatment delayed corneal wound healing via the induction of cholesteryl sulfotransferase in the eye

This study was conducted by Laboratory for Metabolomics, Laboratory for Intestinal Ecosystem and Laboratory for Genotyping Development, RIKEN Center for Integrative Medical Sciences (IMS). They demonstrated antibiotics treatment using C57BL/6J wild-type mice. Antibiotic-treated mice were generated by administering a cocktail of four antibiotics (ampicillin, neomycin, vancomycin and metronidazole) via drinking water to wild-type mice for 4 weeks, followed by topical application for 1 week. Male mice aged 8-14 weeks were used for in vivo animal studies. All mouse studies were performed in compliance with RIKEN-approved protocols (29-009-4) and institutional guidelines. The eye samples were obtained in 2024 and we collected gut microbiome data by 16S rRNA gene amplicon sequencing.