Minimum inhibitory concentration (MIC) of the top 15 synthetic compounds against B. cenocepacia K56-2, the Survival100 (Surv100) concentration in C. elegans and the Surv100/MIC ratio.

aMIC was determined using the standard microtitre broth dilution method as specified by CLSI guidelines [32] in MHB with cation supplementation and a final inoculum of 105 CFU/mL. The concentration range of the test compounds for the MIC is listed in S5 Table.b The Surv100 was defined as the highest soluble compound concentration that results in 100% survival (SURV100) of C. elegans within a 24 hour time frame. The Surv100 assay was conducted as an LKA where 5 to 10 OP50-fed worms per well were exposed to each compound, serial diluted from its highest soluble concentration, for 24 hours at 25°C. The % survival was determined and the ratio of Surv100/MIC was calculated. The compounds with a ratio of 1 and greater were used in the in vivo antibiotic activity assay.Minimum inhibitory concentration (MIC) of the top 15 synthetic compounds against B. cenocepacia K56-2, the Survival100 (Surv100) concentration in C. elegans and the Surv100/MIC ratio.