mRNA expression profiling A549 lung cancer cells following PPARGC1A suppression

PPARGC1A oppositely regulates cancer metastasis in melanoma, breast, and pancreatic cancer; however, little is known about its impact on lung cancer metastasis. We generated gene-expression profile of control and PPARGC1A suppressed A549 cells, a lung adenocarcinoma cell line that expresses moderate levels of PPARGC1A to investigate the role of this gene in lung cancer metastasis. Lentivirus expressing shRNA against PPARGC1A or control was infected into A549 lung cancer cells. The infected cells were selected with puromycin for 5 days, and then total RNA was used to analyze gene expression profiling.