Small RNA and mRNA expression profiling during axolotl forelimb regeneration

Previous studies of appendage regeneration in the axolotl have shown that multiple genetic programs are modulated through regulatory factors. MicroRNAs are short highly conserved non-coding genes that suppress expression of target genes and thereby control multiple genetic programs. Given their important regulatory roles and evolutionary conservation, we hypothesize that microRNAs define a conserved genetic regulatory circuit important for appendage regeneration. We characterized microRNA expression during Axolotl forelimb regeneration using small RNA sequencing. The same samples were assayed for mRNA expression using mRNA sequencing.

过往针对美西钝口螈（axolotl）附肢再生的相关研究显示，多种遗传程序可通过调控因子实现表达调控。微小RNA（MicroRNAs）是一类短小且高度保守的非编码基因，可通过抑制靶基因的表达来调控多种遗传程序。鉴于其重要的调控作用与进化保守性，本研究提出假说：微小RNA构成了对附肢再生至关重要的保守遗传调控回路。本研究采用小RNA测序（small RNA sequencing）技术，解析了美西钝口螈前肢再生过程中的微小RNA表达谱；同时利用mRNA测序（mRNA sequencing）技术，对同一批样本的mRNA表达水平进行了检测。

搜集汇总

背景与挑战

背景概述

该数据集通过RNA-Seq和miRNA-Seq技术，对墨西哥钝口螈（Ambystoma mexicanum）前肢再生过程中的小RNA和mRNA表达进行测序分析，旨在探究微RNA在肢体再生中的调控作用。数据集包含8个样本，覆盖再生不同时间点（0、3、6、14天），总数据量约10.03 GB，为研究再生生物学提供了转录组层面的关键数据。