Microarray gene expression analysis of white and brown adipose tissue depots from mice exposed to cold or room temperature.

In the last decades, adipose tissue has been defined to play a central role in the control of energy balance. Furthermore, the proven endocrine and thermogenic functions of adipocytes have renewed interest in the study of this tissue, as the activation of non-shivering thermogenesis may hold great potential for the future treatment of obesity and T2D. Cold exposure has defined to activate this process. Here, the transcriptomic response of epididymal and inguinal white adipose depots (eWAT and iWAT, respectively) as well as that of the interscapular brown adipose depot (iBAT) of C57Bl/6 mice either exposed to 22ºC or 4ºC for the period of 4 days were examined. This in-detail study of the adaptation of each depot to cold exposure has allowed unraveling depot-specific regulatory molecular mechanisms. A total number of 8 mice were divided in two cohorts of 4 mice. The first group was housed at room temperature and the second group at cold exposure. Then, iBAT, iWAT and eWAT samples were collected from each mice. Total samples 24.