Detection and prevalence of Epstein-Barr virus (EBV) infection in patients with diffuse large B-cell lymphoma (DLBCL) – raw data

The aim of the scientific activity was to apply sensitive molecular biology methods for the detection and quantification of Epstein–Barr virus (EBV) genetic material in biological samples obtained from patients with diffuse large B-cell lymphoma (DLBCL). Raw data generated within the project have been deposited, including:(i) raw data obtained using droplet digital PCR (ddPCR) for quantitative detection of EBV DNA and RNA in samples from lymphoma patients, targeting the BamHI-W fragment of the EBV genome, with simultaneous detection of the human reference gene RPP30. The dataset also includes ddPCR results for the detection of viral mRNA transcripts of EBER1, LMP1, and EBNA2, analyzed together with the human reference gene GAPDH. The deposited data comprise output data generated by the ddPCR platform;(ii) microscopic images documentation of the detection of viral EBER1/2 transcripts in tumor tissue from patients with DLBCL and Hodgkin lymphoma (HL), obtained using in situ hybridization (EBER-ISH). The dataset contains raw microscopy PNG images;(iii) visualization of amplified fragments of the EBV EBNA2 and LMP1 genes obtained by automated capillary electrophoresis using the QIAxcel system in samples from lymphoma patients. The deposited files include graphical outputs (PNG images) showing the separation of PCR products and their corresponding amplicon sizes;(iv) raw chromatograms (AB1 files) from Sanger DNA sequencing of PCR amplification products of the EBV EBNA2 and LMP1 genes in samples from lymphoma patients. The chromatogram files can be opened and analyzed using, for example, free Chromas software version 2.6.6, available from the Technelysium website.This study was fully funded by the National Science Centre, Poland (grant no. 2024/08/X/NZ7/01767).The attached files contain the raw data used for the analyses.