scRNA-seq of index-sorted myeloid and lymphoid progenitor cells and differentiated pDCs

Nine different cell types (common dendritic progenitor (CDP), pre-conventional dendritic cell (pre-cDC), common dendritic progenitor (CDPr, according to Rodrigues et al., but this population was flawed in sorting purity), Flt3+ CD11c+ Siglec-H+ CCR9-low B220-low progenitor cell (lo-lo), Flt3 + CD11c+ Siglec-H+ CCR9-low B220-high progenitor cell (lo-hi), plasmacytoid dendritic cell (pDC), Ly6D+ lymphoid progenitor (SP), Ly6D+ Siglec-H+ lymphoid progenitor (DP) and common lymphoid progenitor (CLP)) were sorted to allow for analysis of their transcriptomic relation and/or similarity. The pDC_precursor_scvelo.h5ad file is a processed file ready for direct downstream analysis with scvelo.本研究对九种细胞类型进行了分选，以分析其转录组关联及/或相似性，所涉细胞类型包括：普通树突状细胞祖细胞（common dendritic progenitor, CDP）、经典树突状细胞前体细胞（pre-conventional dendritic cell, pre-cDC）、普通树突状细胞祖细胞（CDPr，据Rodrigues等人的研究，但该群体的分选纯度存在缺陷）、Flt3+CD11c+Siglec-H+CCR9低表达、B220低表达祖细胞（lo-lo）、Flt3+CD11c+Siglec-H+CCR9低表达、B220高表达祖细胞（lo-hi）、浆细胞样树突状细胞（plasmacytoid dendritic cell, pDC）、Ly6D+淋巴样祖细胞（SP）、Ly6D+Siglec-H+淋巴样祖细胞（DP）以及普通淋巴样祖细胞（common lymphoid progenitor, CLP）。pDC_precursor_scvelo.h5ad文件为已完成预处理的数据集，可直接用于scvelo的下游分析。