Amacrine cells differentially balance zebrafish colour circuits in the central and peripheral retina

In vertebrate vision, the feature-extracting circuits of the inner retina are driven by photoreceptors whose outputs are already pre-processed. In zebrafish, for example, outer retinal circuits split “colour” from “greyscale” information across all four cone-photoreceptor types. How does the inner retina process this incoming spectral information while also combining cone signals to shape new greyscale functions?
We address this question by imaging the light-driven responses of amacrine cells (ACs) and bipolar cells (BCs) in larval zebrafish, in the presence and pharmacological absence of inner retinal inhibition. We find that amacrine cells exert distinct effects on greyscale processing depending on retinal region, as well as contributing to the generation of colour opponency in the central retina. However, in the peripheral retina amacrine cells enhanced opponency in some bipolar cells while at the same time suppressing pre-existing opponency in others, such that the net change in the..., All the imaging data was collected under two-photon microscope, the data was then further processed using ImageJ or custom-written script in Igor Pro., the *.h5 files can be opened by any software support HDF5 format.