PAN-INTACT enables isolation of lineage-specific nuclei from fibrous tissues

PAN-INTACT method can be used to isolate cell type specific nuclei from fibrous mouse organs, which are particularly problematic. It is broadly applicable for profiling the transcriptional and epigenetic landscape of specific cell types. We envision that our method can be used to systematically probe mechanistic details of cell type-specific functions within individual organs of intact mice. We demonstrate successful isolation of cell type-specific nuclei from mouse heart and kidney. As a proof of generating high quality epigenome maps from specific cell-types in mouse heart, we used nuclei from PAN-INTACT followed by MAN-IP to construct ATAC-Seq libraries in the study.