Assessing efficiency of single cell population by Drop-seq using cell mixture of epithelial and myeloid cells.

A lung epithelial cell line was mixed with monocytes/marcophages, and then cells were separated using specific antibodies and/or Drop-seq. PC9 is a non-small cell lung carcinoma (NSCLC) cell line. Myeloid lineage cell line U937 are known to differentiate along monocyte/marcophage lineage by TPA. Using Drop-seq, we were able to clearly distinguish PC9 from U937 cells in the mixture. The separation by Drop-seq was correlated with the separation by cell type-specific antibodies. We were able to identify specific biomarkers, as preferentially expressed genes, for each cell population. The results of our study addresses the quality of cell separation by Drop-seq. Overall design: efficincy of cell separation using single cell RNA-seq