An Anterior Second Heart Field Enhancer Regulates the Gene Regulatory Network of the Cardiac Outflow Tract

To identify a functional gene regulatory network that orchestrate transcriptional programs to direct proper outflow tract development, we generated transgenic mice harboring a 226bp-deletion within a GATA-dependent, highly conserved cardiac enhancer region. We then performed gene expression profiling analysis using data obtained from RNA-seq of outflow tract in wildtype and homozygous mutant littermates at embryonic day 12.5. Comparative gene expression profiling analysis of RNA-seq data for Nkx2-5 anterior second heart field enhancer mutants and wildtype littermates.为鉴定可协调转录程序以指导正常流出道发育的功能性基因调控网络，我们构建了在GATA依赖性、高度保守的心脏增强子区域内携带226bp缺失的转基因小鼠。随后，我们针对野生型与纯合突变型同窝仔鼠在胚胎第12.5天的流出道组织开展RNA测序（RNA-seq），并基于所得测序数据完成基因表达谱分析。本研究同时对Nkx2-5前第二心场增强子突变小鼠与野生型同窝仔鼠的RNA-seq数据进行对比基因表达谱分析。