Zebrafish Purkinje cells electrical properties

Title
Functionally-distinct Purkinje cell types show temporal precision in encoding locomotion

Authors
Weipang Chang, Andrea Pedroni, Victoria Hohendorf, Stefania Giacomello,  Masahiko Hibi, Reinhard W. Köster and Konstantinos Ampatzis

Abstract
Purkinje cells, the principal neurons of cerebellar computations, are believed to comprise a uniform neuronal population of cells, each with similar functional properties. Here, we show an undiscovered heterogeneity of adult zebrafish Purkinje cells, revealing the existence of anatomically and functionally distinct cell types. Dual patch-clamp recordings showed that the cerebellar circuit contains all different Purkinje cell types that cross-communicate extensively using chemical and electrical synapses. Further activation of spinal central pattern generators (CPGs) revealed unique phase-locked activity from each Purkinje cell type during the locomotor cycle. Thus, we show intricately organized Purkinje cell networks in the adult zebrafish cerebellum that encode the locomotion rhythm differentially, and we suggest that these organizational properties may also apply to other cerebellar functions.

标题
功能区分的浦肯野细胞类型在编码运动过程中展现时间精确性
作者
查伟邦，安德烈亚·佩德罗尼，维多利亚·霍亨多夫，斯蒂法尼亚·贾科梅洛，麻生弘行，赖因哈德·W·科斯特，康斯坦丁诺斯·安帕齐斯
摘要
浦肯野细胞，作为小脑计算的主要神经元，被普遍认为构成一个具有相似功能特性的均匀神经元群体。在本研究中，我们揭示了成年斑马鱼浦肯野细胞的未发现异质性，发现了在解剖学和功能上有所区别的细胞类型。双电极片记录显示，小脑回路包含所有不同类型的浦肯野细胞，这些细胞通过化学和电突触广泛进行交叉通信。进一步激活脊髓中枢模式发生器（CPGs）揭示了在运动周期中每种浦肯野细胞类型独特的相位锁定活动。因此，我们展示了成年斑马鱼小脑中复杂组织的浦肯野细胞网络，这些网络以不同方式编码运动节奏，并提出这些组织特性可能也适用于其他小脑功能。