Transcriptomics of impaired hypoxic recovery of L2HGDH mutants

We found that L2HGDH mutants, in addition to accumulating L-2HG, are profoundly sensitive to hypoxic conditions (1% O2), in contrast to normal or heterozygote control flies which can survive and recover from hypoxia even after 12 hours of exposure. Here, we sought to investigate the trascriptomic changes associated with the response to hypoxia and recovery in both control and mutant backgrounds, with emphasis on the contrast between the normal and defective response at each time point. Control (heterozygous: L2hgdh[12]/[+]) and mutant (L2hgdh[12]/[14]) adult flies were subjected to hypoxia (1% O2, 12h), then collected during a timecourse of normoxic recovery (0h, 1h, 12h) for RNA extraction and RNA-seq analysis.