RNA-sequencing of testes from control and Hormad1&2SFB/SFB mice

To examine whether Hormad1&2SFB/SFB (dsfb) male mice has defect in the meiotic sex chromosome inactivation (MSCI) process or not, three pairs of P19 testes were collected from each strain and mRNA-sequencing was performed. The data revealed that the transcription of X and Y chromosomes, but not autosomes, were specifically elevated. Therefore, MSCI was defective in dsfb spermatocytes. Three pairs of PD19 testes from different mice were isolated and mRNA-sequencing was performed on the samples.为探究Hormad1&2SFB/SFB（dsfb）雄性小鼠是否存在减数分裂性染色体失活（meiotic sex chromosome inactivation, MSCI）缺陷，本研究从各品系小鼠中采集3对出生后第19天（P19）的睾丸组织，开展mRNA测序实验。测序结果显示，X染色体与Y染色体的转录活性特异性上调，而常染色体无此变化。由此证实，dsfb小鼠的精母细胞存在MSCI缺陷。此外，本研究还从不同小鼠体内分离得到3对出生后第19天（PD19）的睾丸组织，并对其样本开展mRNA测序。