Interactions between reactive oxygen species stress and brassinosteroid hormone signaling in Arabidopsis seedlings

We were interested in investigating the transcriptome responses to exogenous applications of brassinosteroid hormone when Arabidopsis seedlings are pre-stressed with a reactive oxygen species, hydrogen peroxide. We were interested in seeing which subsets of BR-responsive gene transcripts were most affected and how BR-responsive gene transcripts responded to increasing concentrations of hydrogen peroxide both as a whole and individually. Overall design: Liquid culture Arabidopsis seedlings are grown under standard conditions. Hydrogen peroxide is added at various concentrations to pre-stress the seedlings. Following this pretreatment, the seedlings are then treated with brassinosteroid (BR) hormone (epi-brassinolide, BL). Following this treatment, seedlings are harvested and total RNA is extracted for genome-wide transcriptome analysis.

本研究旨在探究当拟南芥幼苗在接触反应性氧化物种——过氧化氢进行预处理时，外源施用 brassinosteroid 激素对转录组的影响。我们关注的是哪些 BR 响应基因转录本子受到最大影响，以及这些基因转录本如何整体及个别地响应不同浓度的过氧化氢。总体设计如下：将拟南芥幼苗在标准条件下进行液体培养。以不同浓度添加过氧化氢以预处理幼苗。预处理后，对幼苗施以 brassinosteroid 激素（如 epi-brassinolide，BL）。施用激素后，收获幼苗并提取总 RNA 以进行全基因组转录组分析。