In vitro properties of patient serum predict clinical outcome after high dose rate brachytherapy of hepatocellular carcinoma

Tumor recurrence after local tumor ablation, including high-dose rate brachytherapy (HDR‑BT), represents a substantial challenge in hepatocellular carcinoma (HCC) treatment. This study aimed to investigate whether induced factors that appear in patient serum after HDR-BT alter HCC growth in vitro, and whether this correlates with outcome. In total, 23 HCC patients [Barcelona clinic liver cancer (BCLC) stage A or B] were treated by HDR‑BT (1×15 Gy) and classified as responders in case of no progression within 6 months and no diffuse systemic progression within 2 years (n=12), or non-responders with recurrence within 6 months and/or diffuse systemic tumor progression or extrahepatic disease within 2 years (n=11). Patient serum was obtained at baseline and 48 h post-procedure. Two hepatoma cell lines (HepG2, Huh7) were incubated for 72 h in the presence of 20% serum. BrdU incorporation was assessed for serum incubation at baseline and 48 h post-HDR‑BT. BrdU incorporation post-HDR‑BT compar..., , , # Data from: In vitro properties of patient serum predict clinical outcome after high dose rate brachytherapy of hepatocellular carcinoma

[https://doi.org/10.5061/dryad.9p8cz8wt0](https://doi.org/10.5061/dryad.9p8cz8wt0)

## Description of the data and file structure

Sheet 1 (‘BrdU incorporation HepG2, Huh7’): Shown are basic characteristics of the investigated patients as well as the BrdU incorporation of HepG2 and Huh7 in the serum incubation experiments. Briefly, serum of responders and non-responders to high dose rate brachytherapy (HDR-BT) was collected before (pre) and 48 h after (post) the procedure. Patient serum was used to incubate HepG2 and Huh7 cells in vitro for a period of 72 h. After incubation, the incorporation of BrdU (5-bromo-2'-deoxyuridine) was determined in HepG2 and Huh7 cells. The BrdU incorporation was evaluated as the BrdU positive area in relation to the DAPI positive area in the entire high-power field. For each cell line, the experiments were carried out t..., The authors confirm that all analyses were performed on anonymized data. To further ensure de-identification, variables such as age and other potentially identifying informatino were removed from the provided dataset.