RNAseq analysis of genes differentially expressed in SMMC7721 cells with SNRPE knockdown

Hepatocellular carcinoma (HCC) is a malignant tumor with poor prognosis and lack of effective treatment targets is the major challenge in treating HCC. Previous studies have revealed significant changes in spliceosome-related genes in HCC. The unique expression pattern in tumor cells makes oncofetal proteins ideal candidate targets for cancer diagnosis and treatment. Therefore, we focused on the differentially expressed splicing factors in embryonic liver development and HCC. We identified an oncofetal splicing factor, small nuclear ribonucleoprotein polypeptide E (SNPRE).In order to better understand the effect of SNRPE in HCC, we knocked down SNRPE using siRNA (siSNRPE) assessed the gene expression pattern by RNA-Seq. Six samples obtained from smmc7721 cells including negative control (NC) and siSNRPE group were analyzed. Three independent samples were analyzed for each group.