Metabolite-mediated trophic interactions: Long-term responses of Thalassiosira weissflogii to high CO₂ and warming have cascading effects on consumer metabolism

Overview

This dataset contains measurements of metabolite contents and physiological parameters of the marine diatom Thalassiosira weissflogii and associated feeding experiments on clams. The dataset was generated as part of a study examining the effects of high CO2, warming, and their combination on T. weissflogii and downstream impacts on clams.

Files Included

File #1: Tw-meta_intensity_all_noknn.xls

Description: Absolute contents of 164 metabolites in T. weissflogii under four experimental conditions.

Columns:

Compound_ID: Unique identifier for each metabolite.

Name: Common name of the metabolite.

Group: Classification of the metabolite (e.g., amino acids, fatty acids, organic acids).

HMDB: Human Metabolome Database annotation for the metabolite.

Control_1, Control_2, Control_3: Replicates for ambient condition.

CO2_1, CO2_2, CO2_3: Replicates for high CO2 condition.

Tem_1, Tem_2, Tem_3: Replicates for warming condition.

TC_1, TC_2, TC_3: Replicates for high CO2 + warming condition.

QC_1, QC_2: Quality control samples.

Units: nmol/g.

File #2: Clams-meta_intensity_all_noknn.xls

Description: Metabolite contents in clams after feeding on T. weissflogii cultured under four experimental conditions for three weeks.

Structure: Same as File #1.

Units: nmol/g.

File #3: Physiological responses of T. weissflogii.xlsx

Description: Physiological measurements of T. weissflogii under four experimental conditions. Contains three sheets:

Sheet 1: Particulate organic carbon (POC) content (pg/cell).

Sheet 2: Particulate nitrogen (PON) content (pg/cell).

Sheet 3: Specific growth rate (μ, d⁻¹).

Conditions:

LCLT: Ambient.

LCHT: Warming.

HCLT: High CO2.

HCHT: High CO2 + warming.

Units: POC and PON in pg/cell; growth rate in d⁻¹.

Relationships Between Files

File #1 and File #2: Provide complementary insights into the effects of environmental conditions on T. weissflogiimetabolites and subsequent effects on clams.

File #3: Contains physiological context for interpreting metabolic changes observed in File #1 and their potential impact on clams in File #2.

How to Use the Data

Refer to File #1 for metabolic profiling of T. weissflogii.

Use File #2 to explore the downstream impacts of T. weissflogii metabolites on clam metabolism.

Use File #3 for physiological measurements of T. weissflogii under different conditions to understand growth and nutrient dynamics.