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QTL Guided Metabolic Engineering of a Complex Trait. Saccharomyces cerevisiae

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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA322647
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We developed an integrated approach for QTL guided metabolic engineering based on high resolution QTL mapping, bulk reciprocal hemizygosity analysis, and CRISPR/Cas9 allele replacements. In this project we studied the genetic basis of hydrolysate tolerance in a cross between the laboratory strain S288C and the industrial-derived strain JAY291.
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2016-05-24
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