Profiling of chromatin accessibility and identification of general cis-regulatory mechanisms that control two ocular lens differentiation pathways

We performed ATAC-seq for microdissected lens epithelium and fiber from E14.5 and P0.5 mouse lenses.Through investigating dynamics of chromatin changes during mouse lens fiber and epithelium differentiation, we identified spatial-temporal differential accessible regions and the enriched known and novel motifs. We also discovered some novel and known enhancers for the transcription factors and structural genes that are important in lens development. Additionally, a cross examination of Pax6 ChIP-seq with ATAC-seq data revealed novel Pax6 binding mechanisms. Overall design: Examination of chromatin profiles in lens epithelium and fiber at E14.5 and P0.5 mouse lenses