Jennifer Bestman, Holly Cline (2011) CIL:36849, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #10 (second time-lapse set, 20 minutes) of a time lapse series contained with in this image group. It shows neurons from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.

The images were acquired with 10 minute intervals and show morphological plasticity of the dendritic arbor with branch additions and retractions.

These cells were transfected with in vivo electroporation of plasmid DNA encoding eYFP ~24 hours before the first image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

Images were acquired every 10 minutes for 1 hour. After 60 minutes, the animal was placed in a 50 microMolar anisomycin solution for ~1.5 hours. A second time lapse series (10 minute interval for 60 minutes) was then acquired.

本图像组所包含的时间序列影像之第十帧（第二次时间序列集合，时长20分钟），呈现了白化非洲爪蟾（Xenopus laevis）中枢神经系统视束神经元的形态可塑性变化，具体处于第47发育阶段。这些图像以10分钟的时间间隔采集，揭示了树突丛的分支增长与回缩等形态学上的可塑性变化。在采集首张图像的前约24小时内，通过对编码增强型黄色荧光蛋白（eYFP）的质粒DNA进行活体电穿孔转染处理，对所研究细胞进行基因改造。实验中，对完好的活体蝌蚪进行麻醉，置于载玻片下方，并通过自制的双光子显微镜直接透过其透明皮肤进行成像。图像以10分钟的时间间隔连续采集1小时，共计60分钟。随后，将动物置于50微摩尔异染霉素溶液中浸泡约1.5小时。接着，采集了第二组时间序列影像（同样以10分钟的时间间隔采集，时长60分钟）。