Mouse Bone-marrow Stromal Cell Lines Genetic Expression Comparison. Mus musculus
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA261523
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资源简介：
OP9 stromal cells are strong inducers of mesoendodermal differentiation of human embryonic stem cells. They acquire inductive function after prolonged post-confluence culture. The experiment is designed to determine the genes specifically expressed in these highly inductive OP9 cells compared to non-confluent OP9 cultures as well as other stromal cell lines (MS5, S17). Overall design: OP9, MS5, and S17 cells were plated on gelatin-coated 10cm dishes in alphaMEM/20%FBS. Cells were collected at confluence (day 4) and after additional 4 days post-confluence culture. Half of the medium was replaced with fresh medium on day 4. Cells were collected by trypsin-EDTA, lysed in TRIReagent and strored at -80C up to 1 month. RNA was isolated and treated with DNase using Ambion RiboPure RNA isolation kit and TURBO DNAfree reagents. One sample was analyzed per condition.
创建时间：
2014-09-19
