Mechanism of circADD2 as ceRNA in Childhood Acute lymphoblastic leukemia

In the present study, we found that circADD2 was down-regulated in ALL tissues and cell lines. Overexpression of circADD2 inhibited cell proliferation and promoted apoptosis both in vitro and in vivo. Additionally, we found that miR-149-5p may increase the expression of the target gene AKT2 through the mechanism of RNAa, and the level of AKT2 can be reversed by circADD2. Briefly, circADD2 could directly sponge miR-149-5p to downregulate AKT2 expression. circADD2 was selected by microarray assay and confirmed by qRT-PCR; in vitro effects of circADD2 were determined by CCK8 and flow cytometry; while mice subcutaneous tumor model was designed for in vivo analysis. RNA immunoprecipitation and dual-luciferase assay were applied for mechanistic study. Protein levels were examined by Western blot assay.