RNA sequencing data of Ssu72 Wild Type and Knock-Out MEFs, hepatocytes and ES cells

To investigate how mammalian Ssu72 regulated gene expression, we performed RNA-Seq analyses of Ssu72 in three different cell types depending on their proliferation capabilities: embryonic stem (ES) cells (highly proliferating), primary murine embryonic fibroblasts (MEFs) (moderate proliferating), and hepatocytes (almost quiescent). Ssu72 deletion was induced by infection with Cre recombinase adenovius (Ad-Cre). Infection of ESf/f, MEFf/f, and hepatocytef/f with Ad-Cre resulted in efficient reduction of Ssu72 levels. Depletion of Ssu72 resulted in downregulation or upregulation of large subsets of genes in three different cell types. MEFs, hepatocytes and ES cells mRNA profiles of 6-weeks old wild type (WT) and Ssu72 knock-out (KO) mice were generated by deep sequencing, in duplicate or triplicate, using Illumina GAIIx.