Image repository from : Subcellular nanoparticle trafficking investigated with label-free, live cell imaging

Nanoparticle drug delivery systems have significant potential to transform
precision medicine due to their ability to encapsulate a wide range of
cargos that can be packaged in various types of carriers. Delivery
kinetics can also be modified by altering the surface chemistry of the
nanoparticle carrier. However, a gap remains in our understanding of the
cellular mechanisms underlying nanoparticle uptake and subcellular
trafficking kinetics. A comprehensive understanding of nanoparticle-cell
interactions considering both the nanocarrier and the drug cargo has been
challenging, partly due to technological limitations. Here, we present a
robust long-term imaging workflow as a novel tool to study live-cell
dynamics and nanoparticle delivery. We show how the integration of
holography, tomography, and fluorescence microscopy enhances the capacity
to study living, adherent cells in vitro label-free with further
applications in assessing nanoparticle uptake and delivery kinetics over
time. We also introduce a method of characterizing kinetic data from a
library of lipid- and polymer-based nanoformulations without the need for
cellular labels. This application of dynamic fluorescent holotomography as
a new method to investigate nanoparticle uptake and cargo delivery
highlights the expanding utility of multimodal, label-free, live imaging
techniques.