Rescue of infectious FMDV from FMDV RNA.
收藏Figshare2015-12-02 更新2026-04-29 收录
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Different levels of FMDV RNA (expressed as the Ct value from the standard assay) were introduced into BHK cells by electroporation and added to monolayers of BTY cells. The cells were examined each day for virus production (as indicated by generation of CPE, ++ indicates partial or +++ complete cell lysis) For the second passage, 200 µl of virus harvest was added to a fresh monolayer of BTY cells. Neg indicates no CPE while the (?) indicates uncertainty due to the presence of many floating cells generated by the electroporation.
创建时间：
2015-12-02
