Cicer arietinum Transcriptome or Gene expression

Chickpea seeds (C. arietinum var. Pusa 362) procured from the Indian Agricultural Research Institute, New Delhi, India, were grown in the fields of NBRI in winters from December to March. Newly emerged and fully expanded bi-pinnate leaves of eight week old chickpea plants were wounded with a pair of pointed forceps by rapid and repeated pricking while still on the plant. Just prior to wounding, the tissue was exposed to insect saliva obtained from the oral secretions of H. armigera (spread over the tissue with a soft brush). The wounded tissue was kept for 20 min on the plant, excised, frozen in liquid nitrogen, followed by RNA isolation. The RNA was used for illumina sequencing.