KIT-expressing cells in the mouse fetal liver

Various blood lineages, including lymphoid cells and myeloid cells, are produced from hematopoietic stem cells (HSCs) in the bone marrow. However, HSCs arent developed in the bone marrow during mouse embryogenesis. The first HSCs are found in the aorta-gonad-mesonephros region, the yolk sac, and the fetal liver, although the number is very few at embryonic day (E) 11. Most HSCs are observed in the fetal liver at E12. To understand the specific genes of nascent HSCs, we performed single-cell RNA-seq analysis of KIT-expressing cells sorted from the fetal liver at E12. We identified the nascent HSC population and found some feature genes in those cells. Therefore, our data will help us understand the nascent HSCs. Single-cell RNA-seq analysis of KIT-expressing cells sorted from the mouse fetal liver at E12多种血液谱系，包括淋巴样细胞（lymphoid cells）与髓样细胞（myeloid cells），均由骨髓中的造血干细胞（hematopoietic stem cells, HSCs）分化生成。然而在小鼠胚胎发育阶段，造血干细胞并非在骨髓中产生。首批造血干细胞可在主动脉-性腺-中肾区（aorta-gonad-mesonephros region）、卵黄囊（yolk sac）及胎肝（fetal liver）中被检出，但在胚胎第11天（embryonic day, E11）时其数量极为稀少。至胚胎第12天（E12），胎肝中可观测到绝大多数造血干细胞。为探明新生造血干细胞的特异性基因，我们对胚胎第12天小鼠胎肝中分选获得的KIT阳性细胞开展了单细胞RNA测序（single-cell RNA-seq）分析，成功鉴定出新生造血干细胞群，并在该细胞群中发现了若干特征基因。本数据集将为深入解析新生造血干细胞提供重要数据支撑。本数据集包含对胚胎第12天小鼠胎肝中分选得到的KIT阳性细胞的单细胞RNA测序分析。