Data underlying the chapter: Discovery and synthetic applications of a NAD(P)H-dependent reductive aminase from Rhodococcus erythropolis

This dataset contains data collected during experiments at Delft University of Technology, as Chapter 5 of the dissertation written by Ewald Jongkind. The aim of this work was to find and characterize reductive aminases from ammonia, where we focussed on one enzyme from Rhodococcus with the highest activity. This work was done by searching for new enzymes with EnzymeMiner, express and purify active enzymes and characterize these enzymes. Dataset contains output data from our enzyme search in Excel, images of enzyme purifications, conversions and activities determined with UV-VIS (calculated in Excel), and NMR-spectra of synthesized products. This rough data was uploaded in combination with a draft of the Supporting Information to be submitted for publication.

本数据集包含代尔夫特理工大学（Delft University of Technology）实验过程中采集的原始数据，系Ewald Jongkind所撰写的博士论文的第5章内容。本研究的目标为从氨源中筛选并表征还原氨基化酶，我们重点聚焦于一株来自红球菌属（Rhodococcus）、活性最高的酶。本工作通过EnzymeMiner工具检索新型酶，对活性酶进行表达与纯化，并完成相关酶学表征。数据集包含通过EnzymeMiner检索得到的酶学搜索结果Excel文件、酶纯化过程的成像图谱、经紫外-可见分光光度法（UV-VIS）测定并在Excel中计算得到的酶催化转化率与活性数据，以及合成产物的核磁共振（Nuclear Magnetic Resonance, NMR）谱图。本套原始数据连同待投稿的补充材料草案一同完成上传。