Summary of pheromone experiments in Pseudostaurosira trainorii.

All experiments were performed in triplicate and the results were consistent. Unless stated otherwise, ♂ and ♀ are vegetative cells of male and female clones, respectively; S = sexualized clone where released gametes and gametangia were predominant with a small amount of vegetative cells; F = filtrate, so that F[♀}, F[♂S], etc. are filtrates of females, male sexualized clones, respectively.*♀ gametes were killed by heating to 98°C for 1 h, or leaving Eppendorf tubes under 321 nm UV light (with TFX-20M transilluminator: Life Technologies, Inc, Paisley, UK) for 1 h.